Confirm
Products

Serum-free Medium for Vero Cells

+
Product Description

ToCell® Vero CD is a serum-free, chemically defined, and animal-component-free adherent medium, specifically formulated for Vero cells.

Product Features
● Chemically defined
● Serum-free
● High proliferation rate
● Animal-free components
● No cell acclimation required
 
Product Application

Applicable for large-scale industrial production of vaccines, such as COVID-19, rabies (for human and veterinary use), Japanese encephalitis (JE), hepatitis A, inactivated poliovirus (IPV), EV71, rotavirus, veterinary rabies, and porcine epidemic diarrhea vaccines.

Product

Product Code

Packaging Specifications

Vero SFM

633D

1L、10L、25L、50L、100L、200L、250L、300L

Vero VM (Virus maintenance solution)

632

1L、10L、25L、50L、100L、200L、250L、300L
  • Case Presentation

  • Comparative experiment with commercial culture medium

    		 Continuous passage data of Vero cells in different serum-free cultures

    Cell: Vero cell
    Culture medium: Vero cell serum-free medium (633)/a commercial medium
    Total vaccination amount: 7×106cells
    Pancreatic enzyme: TrypLETM Express Enzyme（1X）GIBCO
    Cultivation conditions: 37℃、5%CO₂、T75 Square bottle (breathable cap)
    Exposure dose: 1/200

    Virus: PV strain

    		 111.jpg

    Continuous production process of rabies virus without serum in 14L DISK reactor

    Continuous production of rabies vaccine through serum-free culture in a 14L DISK fixed bed bioreactor
    222.jpg

    The results showed that TXH-633 serum-free medium was amplified from the cell factory and inoculated into a 14L DISK reactor for continued serum-free culture, followed by inoculation with rabies virus. During the continuous harvesting process of viral antigens, there were 14 days where the harvested antigen levels were greater than 20IU/mL, and the process could be further scaled up to meet the large-scale production needs of rabies vaccines for human or animal use.